MRPL47 Overexpression Lysate

Name: MRPL47 Overexpression Lysate
Brand: Novus Biologicals
SKU: NBL1-13274

Novus Biologicals, part of Bio-Techne | Catalog # NBL1-13274

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for MRPL47 Overexpression Lysate

MRPL47 Transient Overexpression Lysate
Expression Host: HEK293T

Plasmid: RC207645

Accession#: NM_177988

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

TMW

16.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for MRPL47 Overexpression Lysate

Western Blot: MRPL47 Overexpression Lysate [NBL1-13274]

Western Blot: MRPL47 Overexpression Lysate (Adult Normal) [NBL1-13274] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MRPL47.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: MRPL47

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene is immediately adjacent to the gene for BAF complex 53 kDa subunit protein a (BAF53a), in a tail-to-tail orientation. Two transcript variants encoding different protein isoforms have been identified. Transcript Variant: This variant (2) lacks an alternate segment, compared to variant 1, which leads to the use of a downstream start codon. The predicted protein (isoform b) is shorter than isoform a. The predicted ORF of this transcript has not been experimentally confirmed.

Alternate Names

CGI-204, L47mt, MGC45403, mitochondrial ribosomal protein L47, MRP-L47, nasopharyngeal carcinoma metastasis-related 1, Nasopharyngeal carcinoma metastasis-related protein 1, NCM139S ribosomal protein L47, mitochondrial

Gene Symbol

MRPL47

Additional MRPL47 Products

Product Documents for MRPL47 Overexpression Lysate

Product Datasheets

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COA

SDS

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Product Specific Notices for MRPL47 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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