Netrin-4 Overexpression Lysate
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-06541
Key Product Details
Species
Human
Applications
Western Blot
Product Summary for Netrin-4 Overexpression Lysate
Netrin-4 Transient Overexpression Lysate
Expression Host: HEK293T
Plasmid: RC224596
Accession#: NM_021229
Protein Tag: C-MYC/DDK
You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Expression Host: HEK293T
Plasmid: RC224596
Accession#: NM_021229
Protein Tag: C-MYC/DDK
You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Product Specifications
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
TMW
69.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Type
Overexpression
Scientific Data Images for Netrin-4 Overexpression Lysate
![Western Blot: Netrin-4 Overexpression Lysate [NBP2-06541]](https://resources.bio-techne.com/images/products/NTN4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06541-img0001.jpg "Western Blot: Netrin-4 Overexpression Lysate [NBP2-06541]")
Western Blot: Netrin-4 Overexpression Lysate [NBP2-06541]
Western Blot: Netrin-4 Overexpression Lysate (Native) [NBP2-06541] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NTN4.Formulation, Preparation, and Storage
Formulation
RIPA buffer
Concentration
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -80C. Avoid freeze-thaw cycles.
Background: Netrin-4
Netrins have been identified as midline-derived chemoattractants that guide axons to the midline during development. Netrins are antagonized by Slit and Robo proteins which compete for DCC (deleted in colorectal cancer) receptor binding. Netrins also have axon repulsion effects when interacting with the UNC5 (uncoordinated-5) receptor family, either alone or with DCC receptors. Netrins are a laminin-related family of matrix-binding secreted proteins containing an N-terminal laminin gamma-chain-related globular domain (domain VI), three laminin EGF-like repeats, and a C-terminal heparin-binding domain.
Netrin-G1a and Netrin-G2a are members of the GPI-linked Netrin subfamily distantly related to classic Netrins. Membrane-bound G-type Netrins are suggested to promote neurite outgrowth and perhaps impact neuronal plasticity. G-type Netrins are found only in vertebrates. Netrin-G1a and Netrin-G2a contain an N-terminal laminin globular domain (domain VI) followed by 3 laminin EGF-like repeats, and a C-domain with a hydrophobic signal for glycosylphosphatidylinositol (GPI) lipid linkage. The Netrin-G2a molecule also contains a C-terminal heparin binding site.
Alternate Names
beta-Netrin, Netrin4, NTN4
Gene Symbol
NTN4
Additional Netrin-4 Products
Product Documents for Netrin-4 Overexpression Lysate
Product Specific Notices for Netrin-4 Overexpression Lysate
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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