NIH 3T3 CoCl2 treated/Untreated Cell Lysate
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11855
Key Product Details
Applications
SDS-PAGE
Product Specifications
Application Notes
NIH 3T3 whole cell lysate is provided as a Western blot positive control. The lysates are provided as a set of treated and untreated samples. Use 10 ul per lane for a standard mini-gel blot (1.0 mg/ml).
Type
Cell
Subcellular Fraction
CoCl2 treated/Untreated
Formulation, Preparation, and Storage
Preparation Method
NIH 3T3 cells are grown under standard tissue culture conditions until semi-confluent (70-80%). The cells are then treated with or without 100 uM CoCl2 for 4 hours, washed in PBS and directly lysed into 1X Laemmli sample buffer with BME. The lysate is sonicated and tested by Western blot.
Formulation
The protein lysate is prepared in 1x Laemmli sample buffer with BME. Boiling before loading is not necessary.
Concentration
1.0 mg/ml
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: NIH 3T3
Additional NIH 3T3 Products
Product Documents for NIH 3T3 CoCl2 treated/Untreated Cell Lysate
Product Specific Notices for NIH 3T3 CoCl2 treated/Untreated Cell Lysate
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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