PTPRO Overexpression Lysate

Name: PTPRO Overexpression Lysate
Brand: Novus Biologicals
SKU: NBL1-14986

Novus Biologicals, part of Bio-Techne | Catalog # NBL1-14986

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for PTPRO Overexpression Lysate

PTPRO Transient Overexpression Lysate
Expression Host: HEK293T

Plasmid: RC216766

Accession#: NM_030667

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

TMW

135.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for PTPRO Overexpression Lysate

Western Blot: PTPRO Overexpression Lysate [NBL1-14986]

Western Blot: PTPRO Overexpression Lysate (Adult Normal) [NBL1-14986] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PTPRO.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: PTPRO

This gene encodes a receptor-type protein tyrosine phosphatase containing a single intracellular catalytic domain with a characteristic signature motif. The gene product, which has a transmembrane domain, is an integral membrane protein. Several alternatively spliced transcript variants, some of which encode different isoforms of the protein, have been described. These variants exhibit tissue-specific expression. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the longest isoform (a) known to date. The encoded protein has a large extracellular domain containing 8 repeats of a fibronectin type III-like motif. This variant is expressed in the glomerulus of kidney and in brain. In kidney, it may play a role in regulating glomerular epithelial cell (podocyte) structure and/or function. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Alternate Names

EC 3.1.3.48, GLEPP1PTPROt, Glomerular epithelial protein 1, phosphotyrosine phosphatase U2, protein tyrosine phosphatase PTP-U2, Protein tyrosine phosphatase U2, protein tyrosine phosphatase, receptor type, O, PTPase U2, PTP-oc, PTP-U2glomerular epithelial protein-1, PTPU2osteoclastic transmembrane protein-tyrosine phosphatase, receptor-type protein tyrosine phosphatase O, receptor-type tyrosine-protein phosphatase O, R-PTP-O

Gene Symbol

PTPRO

Additional PTPRO Products

Product Documents for PTPRO Overexpression Lysate

Product Datasheets

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COA

SDS

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Product Specific Notices for PTPRO Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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