Raji Whole Cell Lysate
Novus Biologicals, part of Bio-Techne | Catalog # NB820-59466
Key Product Details
Species
Human
Applications
Western Blot
Product Summary for Raji Whole Cell Lysate
We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.
Product Specifications
Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression
Specificity
Total Protein - Human Tumor Cell Line: Raji
Type
Cell
Tissue
Blood Lymph
Protein State
Native
Subcellular Fraction
Whole
Scientific Data Images for Raji Whole Cell Lysate
![Western Blot: Raji Whole Cell Lysate [NB820-59466]](https://resources.bio-techne.com/images/products/Raji-Whole-Cell-Lysate-Western-Blot-NB820-59466-img0002.jpg "Western Blot: Raji Whole Cell Lysate [NB820-59466]")
Western Blot: Raji Whole Cell Lysate [NB820-59466]
Western Blot: Raji Whole Cell Lysate [NB820-59466] - Protein lysate can be validated by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein.![SDS-PAGE: Raji Whole Cell Lysate [NB820-59466]](https://resources.bio-techne.com/images/products/Raji-Whole-Cell-Lysate-SDS-Page-NB820-59466-img0001.jpg "SDS-PAGE: Raji Whole Cell Lysate [NB820-59466]")
SDS-PAGE: Raji Whole Cell Lysate [NB820-59466]
SDS-Page: Raji Whole Cell Lysate [NB820-59466] - Protein lysate shows consistent pattern.Formulation, Preparation, and Storage
Formulation
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
Concentration
5.0 mg/ml
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -80C. Avoid freeze-thaw cycles.
Background: Raji
Additional Raji Products
Product Specific Notices for Raji Whole Cell Lysate
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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