ROBLD3 Overexpression Lysate

Name: ROBLD3 Overexpression Lysate
Brand: Novus Biologicals
SKU: NBL1-12864

Novus Biologicals, part of Bio-Techne | Catalog # NBL1-12864

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for ROBLD3 Overexpression Lysate

ROBLD3 Transient Overexpression Lysate
Expression Host: HEK293T

Plasmid: RC200862

Accession#: NM_014017

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

TMW

13.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for ROBLD3 Overexpression Lysate

Western Blot: ROBLD3 Overexpression Lysate [NBL1-12864]

Western Blot: ROBLD3 Overexpression Lysate (Adult Normal) [NBL1-12864] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ROBLD3.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: ROBLD3

The product of this gene is highly conserved with a mouse protein associated with the cytoplasmic face of late endosomes and lysosomes. The mouse protein interacts with MAPK scaffold protein 1, a component of the mitogen-activated protein kinase pathway. In humans, a mutation in this gene has been associated with a primary immunodeficiency syndrome, and suggests a role for this protein in endosomal biogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

Alternate Names

ENDAP, Endosomal adaptor protein p14, HSPC003, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2, late endosomal/lysosomal adaptor, MAPK and MTOR activator 2, Late endosomal/lysosomal Mp1-interacting protein, MAPBPIPRoadblock domain-containing protein 3, MAPKSP1 adaptor protein, MAPKSP1AP, Mitogen-activated protein-binding protein-interacting protein, mitogen-activated protein-binding protein-interacting protein (MAPBPIP), p14Ragulator2, roadblock domain containing 3, ROBLD3

Gene Symbol

LAMTOR2

Additional ROBLD3 Products

Product Documents for ROBLD3 Overexpression Lysate

Product Datasheets

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COA

SDS

Download SDS

Product Specific Notices for ROBLD3 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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