Selenoprotein W Overexpression Lysate

Name: Selenoprotein W Overexpression Lysate
Brand: Novus Biologicals
SKU: NBP2-10861

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-10861

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for Selenoprotein W Overexpression Lysate

Selenoprotein W Transient Overexpression Lysate
Expression Host: HEK293T

Plasmid: RC208062

Accession#: NM_003009

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

TMW

9.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for Selenoprotein W Overexpression Lysate

Western Blot: Selenoprotein W Overexpression Lysate [NBP2-10861]

Western Blot: Selenoprotein W Overexpression Lysate (Adult Normal) [NBP2-10861] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Selenoprotein W.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: Selenoprotein W

The function of Selenoprotein W is not entirely known, but the presence of the bound glutathione moiety indicates that SEPW1 is thought to function in oxidation-reduction catalysis and may play a role in selenium deficiency disorders such as white muscle disease in sheep and Keshan disease in humans. Recently, overexpression of SEPW1 was shown to be glutathione dependent and was shown to markedly reduce the sensitivity of cell lines to H2O2 cytotoxicity. Selenoprotein W encodes a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein shows highest expression in skeletal muscle and heart, and may be involved in oxidation-reduction reactions. A retroprocessed pseudogene, SEPW1P, has been identified and mapped to chromosome 1p35-34.

Alternate Names

selenoprotein W, selenoprotein W, 1, selW

Gene Symbol

SEPW1

Additional Selenoprotein W Products

Product Documents for Selenoprotein W Overexpression Lysate

Product Datasheets

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COA

SDS

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Product Specific Notices for Selenoprotein W Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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