U11/U12-35K Overexpression Lysate

Name: U11/U12-35K Overexpression Lysate
Brand: Novus Biologicals
SKU: NBL1-17489

Novus Biologicals, part of Bio-Techne | Catalog # NBL1-17489

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for U11/U12-35K Overexpression Lysate

U11/U12-35K Transient Overexpression Lysate
Expression Host: HEK293T

Plasmid: RC208918

Accession#: NM_007020

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

Type

Overexpression

Scientific Data Images for U11/U12-35K Overexpression Lysate

Western Blot: U11/U12-35K Overexpression Lysate [NBL1-17489]

Western Blot: U11/U12-35K Overexpression Lysate (Adult Normal) [NBL1-17489] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for U11/U12-35K.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: U11/U12-35K

The protein encoded by this gene is a homolog of U1-snRNP binding protein. Its N-terminal half contains a RNA recognition motif and the C-terminal half is rich in Arg/Asp and Arg/Glu dipeptides, a characteristic of a variety of splicing factors. This gene is differentially expressed in a variety of human tissues. Multiple alternatively spliced transcript variants have been found for this gene, and they differ in the 5' sequence regions. Transcript Variant: This variant (1) has an alternate segment, which lacks the translation start codon but contains an additional ribosomal binding site (RBS), in addition to the RBS in the 5' coding region, as compared to variant 3. It uses a downstream in-frame start codon and encodes the shorter isoform (a).

Alternate Names

HM1, HM-1, MGC138160, Protein HM-1, small nuclear ribonucleoprotein 35kDa (U11/U12), U1 snRNP-binding protein homolog, U11/U12 small nuclear ribonucleoprotein 35 kDa protein, U11/U12 snRNP 35 kDa protein, U11/U12 snRNP 35K, U11/U12-35K, U1-snRNP binding protein homolog, U1SNRNPBPU1 snRNP binding protein homolog

Gene Symbol

SNRNP35

Additional U11/U12-35K Products

Product Documents for U11/U12-35K Overexpression Lysate

Product Datasheets

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COA

SDS

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Product Specific Notices for U11/U12-35K Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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