Recombinant Human 4-1BB Ligand/TNFSF9 Protein

Name: Recombinant Human 4-1BB Ligand/TNFSF9 Protein
Brand: Novus Biologicals
SKU: NBP2-34959

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34959

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P41273

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all 4-1BB Ligand/TNFSF9 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 184 amino acids corresponding to 4-1BB Ligand/TNFSF9 Source: E. coli

Uniprot ID: P41273

Amino Acid Sequence: REGPELSPDD PAGLLDLRQG MFAQLVAQNV LLIDGPLSWY SDPGLAGVSL TGGLSYKEDT KELVVAKAGV YYVFFQLELR RVVAGEGSGS VSLALHLQPL RSAAGAAALA LTVDLPPASS EARNSAFGFQ GRLLHLSAGQ RLGVHLHTEA RARHAWQLTQ GATVLGLFRV TPEIPAGLPS PRSE

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of 4-1BB Ligand/TNFSF9 as determined by LAL method.

Predicted Molecular Mass

19.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by stimulation of IL-8 production using human PBMC is less than 10 ng/ml, corresponding to a specific activity of > 1.0 x 10^5 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human 4-1BB Ligand/TNFSF9 Protein

SDS-PAGE: Recombinant Human 4-1BB Ligand/TNFSF9 Protein [NBP2-34959]

SDS-Page: Human 4-1BBL/TNFSF9 Protein [NBP2-34959]

Formulation, Preparation and Storage

NBP2-34959

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: 4-1BB Ligand/TNFSF9

41BBL is a member of the tumor necrosis factor (TNF) receptor family. This receptor contributes to the clonal expansion, survival, and development of T cells. In addition, 41BBL expression is found on dendritic cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. CD137 has been shown to interact with TRAF2. The human 41BBL gene codes for a 254 amino acid type II transmembrane containing a 28 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 205 amino acid extracellular domain (ECD). The human 41BBL ECD shares 32 % and 35 % a.a. identity with murine and rat ECD.

Alternate Names

41BB Ligand, CD137L, TNFSF9, 4-1BBL/TNFSF9

Gene Symbol

TNFSF9

UniProt