Recombinant Human Adenylosuccinate Lyase His Protein

Name: Recombinant Human Adenylosuccinate Lyase His Protein
Brand: Novus Biologicals
SKU: NBP1-72514

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72514

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Adenylosuccinate Lyase Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-484 of Human Adenylosuccinate Lyase

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAAG GDHGSPDSYR SPLASRYASP EMCFVFSDRY KFRTWRQLWL WLAEAEQTLG LPITDEQIQE MKSNLENIDF KMAAEEEKRL RHDVMAHVHT FGHCCPKAAG IIHLGATSCY VGDNTDLIIL RNALDLLLPK LARVISRLAD FAKERASLPT LGFTHFQPAQ LTTVGKRCCL WIQDLCMDLQ NLKRVRDDLR FRGVKGTTGT QASFLQLFEG DDHKVEQLDK MVTEKAGFKR AFIITGQTYT RKVDIEVLSV LASLGASVHK ICTDIRLLAN LKEMEEPFEK QQIGSSAMPY KRNPMRSERC CSLARHLMTL VMDPLQTASV QWFERTLDDS ANRRICLAEA FLTADTILNT LQNISEGLVV YPKVIERRIR QELPFMATEN IIMAMVKAGG SRQDCHEKIR VLSQQAASVV KQEGGDNDLI ERIQVDAYFS PIHSQLDHLL DPSSFTGRAS QQVQRFLEEE VYPLLKPYES VMKVKAELCL

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Adenylosuccinate Lyase His Protein

SDS-PAGE: Recombinant Human Adenylosuccinate Lyase His Protein [NBP1-72514]

SDS-Page: Recombinant Human Adenylosuccinate Lyase Protein [NBP1-72514] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-72514

Formulation	20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 40% glycerol, 0.1 M NaCl
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Adenylosuccinate Lyase

Adenylosuccinate lyase, also known as ADSL, is an enzyme that converts adenylosuccinate to AMP and fumarate as part of the purine nucleotide cycle. Defects in ADSL are the cause of adenylosuccinase deficiency (ADSL deficiency). ADSL deficiency is an autosomal recessive disorder characterized by the accumulation in the body fluids of succinylaminoimidazole-carboxamide riboside (SAICA-riboside) and succinyladenosine (S-Ado). Recombinant human ADSL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

Adenylosuccinase, adenylosuccinate lyase, AMPS, ASase, ASL, EC 4.3.2, EC 4.3.2.2

Gene Symbol

ADSL

Additional Adenylosuccinate Lyase Products

Product Documents for Recombinant Human Adenylosuccinate Lyase His Protein

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COA

Product Specific Notices for Recombinant Human Adenylosuccinate Lyase His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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