We have used the antibody NBP1-70997 for immunohistochemistry, and now we need to demonstrate specificity of binding. What product do you recommend? Is the using of product NBP1-77078PEP correct? Can you please send more Information (maybe a protocol), about preparation of working dilution of blocking peptides and about incubations times with primary antibodies??

Name: AGTR-1 Antibody Blocking Peptide
Brand: Novus Biologicals
SKU: NBP1-77078PEP

Yes, you are correct, NBP1-77078PEP would be the appropriate blocking peptide to use. Please see our protocol for peptide blocking.

AGTR-1 Antibody Blocking Peptide

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77078PEP

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

Synthetic

Conjugate

Unconjugated

Applications

Antibody Competition

View all AGTR-1 Proteins and Enzymes »

Product Specifications

Description

A blocking peptide containing 16 amino acids near the center of human AGTR1.

Source: Synthetic

(Accession #: EAW78909)

Purity

N/A

Application Notes

This peptide is useful as a blocking peptide for NBP1-77078. For further blocking peptide related protocol, click here.

Protein / Peptide Type

Antibody Blocking Peptide

Formulation, Preparation and Storage

NBP1-77078PEP

Formulation	PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin.
Preservative	0.02% Sodium Azide
Concentration	0.2 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20C. Avoid freeze-thaw cycles.

Background: AGTR-1

Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. At least four transcript variants have been described for this gene. Additional variants have been described but their full-length nature has not been determined. The entire coding sequence is contained in the terminal exon and is present in all transcript variants.

Long Name

Angiotensin II Receptor Type 1

Alternate Names

AG2S, AGTR1, AT1, AT1AR, AT1BR, AT1R, AT2R1

Gene Symbol

AGTR1

Additional AGTR-1 Products

Product Documents for AGTR-1 Antibody Blocking Peptide

Product Datasheets

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COA

SDS

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Product Specific Notices for AGTR-1 Antibody Blocking Peptide

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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