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Albumin Native Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97049

Novus Biologicals, part of Bio-Techne
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NBP1-97049-5mg

Key Product Details

Accession #

Conjugate

Unconjugated

Applications

Dot Blot, SDS-PAGE

Product Specifications

Description

Native Human Albumin

Source: Normal serum (Accession #:P02768)

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.

Purity

Multi-step

Application Notes

This product has been tested in SDS-Page and can be used in SDS, Western Blotting, ELISA experiments and other immunological assays.

Protein / Peptide Type

Native Protein

Scientific Data Images for Albumin Native Protein

SDS-PAGE: Albumin Native Protein [NBP1-97049]

SDS-PAGE: Albumin Native Protein [NBP1-97049]

SDS-Page: Albumin Native Protein [NBP1-97049] - SDS PAGE of Human Albumin. Lane 1: Non-Reduced Human Albumin. Lane 2: 5 uL Opal Prestained Marker. Lane 3: Reduced Human Albumin. Load: 1 ug per lane. Predicted/Observed size: Non-Reduced at 63 kDa/Observed at 55 kDa; Reduced at 63 kDa. Non-reduced migrates farther on gel due to native albumin's capacity to bind ions, increasing its charge and, therefore, ability to migrate farther down gel.
Dot Blot: Albumin Native Protein [NBP1-97049] - Human Albumin Rhodamine Conjugated. Antigen: Rhodamine Conjugated Human Albumin. Load: 3-fold serial dilution starting at 200 ng. Primary antibody: None. Secondary antibody: None. Block for 1 HR at RT.. Using the Rhodamine format of this antibody.
Dot Blot: Albumin Native Protein [NBP1-97049] - Human Albumin Rhodamine Conjugated. Antigen: Rhodamine Conjugated Human Albumin. Load: 3-fold serial dilution starting at 200 ng. Primary antibody: None. Secondary antibody: None. Block for 1 HR at RT.. Using the Rhodamine format of this antibody.

Formulation, Preparation and Storage

NBP1-97049
Preparation Method This product was prepared from normal serum by a multi-step process which includes delipidation and selective precipitation followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Albumin and anti-Human Serum.
Formulation Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative 0.01% Sodium Azide
Concentration Lyoph
Reconstitution Reconstitute with 500 ul of deionzied water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C.

Background: Albumin

Human albumin or serum albumin is encoded by the ALB gene and is the most abundant plasma protein in mammals. Human albumin is essential for maintaining the osmotic pressure needed for proper distribution of body fluids between intravascular compartments and body tissues. Human albumin also acts as a plasma carrier by non-specifically binding several hydrophobic steroid hormones and as a transport protein for hemin and fatty acids. Too much serum albumin in the body can be harmful.

Alternate Names

ALB

Gene Symbol

ALB

UniProt

Additional Albumin Products

Product Documents for Albumin Native Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Albumin Native Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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