Recombinant Mouse AlphaB Crystallin/CRYAB Protein

Name: Recombinant Mouse AlphaB Crystallin/CRYAB Protein
Brand: Novus Biologicals
SKU: NBC1-18376

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-18376

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Scientific Data
Preparation & Storage
Background
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Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

SDS-PAGE

View all AlphaB Crystallin/CRYAB Proteins and Enzymes »

Product Specifications

Description

An un-tagged recombinant protein corresponding to the amino acids 1-175 of Mouse AlphaB Crystallin/CRYAB

Source: E.coli

Amino Acid Sequence: MDIAIHHPWI RRPFFPFHSP SRLFDQFFGE HLLESDLFST ATSLSPFYLR PPSFLRAPSW IDTGLSEMRL EKDRFSVNLD VKHFSPEELK VKVLGDVIEV HGKHEERQDE HGFISREFHR KYRIPADVDP LTITSSLSSD GVLTVNGPRK QVSGPERTIP ITREEKPAVA AAPKK

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse AlphaB Crystallin/CRYAB Protein

SDS-PAGE: Recombinant Mouse AlphaB Crystallin/CRYAB Protein [NBC1-18376]

SDS-Page: Recombinant Mouse AlphaB Crystallin/CRYAB Protein [NBC1-18376] - Crystallin alpha B, 20kDa (175aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Formulation, Preparation and Storage

NBC1-18376

Formulation	20 mM Tris-HCl buffer (pH 8.0), 10 % glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AlphaB Crystallin/CRYAB

Alpha crystallins are composed of two gene products, alpha-A and alpha-B for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20). They act as molecular chaperones and hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha-crystallins are an autokinase activity and participation in the intracellular architecture. CRYAB is expressed widely in many tissues and organs. Recombinant CRYAB was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

CRYA2, CRYAB, CTPP2, HSPB5

Gene Symbol

CRYAB

Additional AlphaB Crystallin/CRYAB Products

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COA

Product Specific Notices for Recombinant Mouse AlphaB Crystallin/CRYAB Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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