Recombinant Human Amphiregulin Protein

Name: Recombinant Human Amphiregulin Protein
Brand: Novus Biologicals
SKU: NBP2-35055

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35055

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P15514

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Amphiregulin Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 98 amino acid residues corresponding to Amphiregulin Source: E. coli

Uniprot ID: P15514

Amino Acid Sequence: SVRVEQVVKP PQNKTESENT SDKPKRKKKG GKNGKNRRNR KKKNPCNAEF QNFCIHGECK YIEHLEAVTC KCQQEYFGER CGEKSMKTHS MIDSSLSK

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of Amphiregulin as determined by LAL method.

Predicted Molecular Mass

11.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Amphiregulin Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is between 5-10 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Amphiregulin Protein

SDS-PAGE: Recombinant Human Amphiregulin Protein [NBP2-35055]

SDS-Page: Recombinant Human Amphiregulin Protein [NBP2-35055]

Formulation, Preparation and Storage

NBP2-35055

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Amphiregulin

Amphiregulin is an EGF related growth factor and was originally isolated from the conditioned media of a PMA-treated MCF-7 human breast carcinoma cell line. It is mainly expressed numerous carcinoma cell lines and the epithelial cells of various human tissues including colon, stomach, breast, ovary, kidney, etc. Synthesized as a transmembrane protein, Amphiregulin's extracellular domain is proteolytically processed to release the mature protein. There are 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds essential for biological activity. Amphiregulin signals through the EGF/TGF-a receptor, and stimulates growth of keratinocytes, epithelial cells and some fibroblasts. It also inhibits the growth of certain carcinoma cell lines. Mutations in this encoded protein are associated with a psoriasis-like skin phenotype.

Alternate Names

AREG

Gene Symbol