APAF-1 Antibody Blocking Peptide

Name: APAF-1 Antibody Blocking Peptide
Brand: Novus Biologicals
SKU: NBP1-77000PEP

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77000PEP

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Technical Data
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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

Synthetic

Conjugate

Unconjugated

Applications

Antibody Competition

View all APAF-1 Proteins and Enzymes »

Product Specifications

Description

A blocking peptide containing 17 amino amino terminus of human Apaf1.

Source: Synthetic

(Accession #: AAC51678)

Purity

N/A

Application Notes

This peptide is useful as a blocking peptide for NBP1-77000. For further blocking peptide related protocol, click here.

Protein / Peptide Type

Antibody Blocking Peptide

Scientific Data Images for APAF-1 Antibody Blocking Peptide

Western Blot: APAF-1 Antibody Blocking Peptide [NBP1-77000PEP]

Western Blot: APAF1 Peptide [NBP1-77000PEP] - Apaf1 peptide is used for blocking the activity of Anti-Apaf1. Incubating the peptide with equal volume of antibody for 30 min at 37C usually completely blocks the antibody activity in Western blotting.

Formulation, Preparation and Storage

NBP1-77000PEP

Formulation	PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin.
Preservative	0.02% Sodium Azide
Concentration	0.2 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20C. Avoid freeze-thaw cycles.

Background: APAF-1

Apaf 1 (apoptosis protease-activating factor-1) is a key regulator of the mitochondrial apoptotic pathway (reviewed in Campioni et al, 2005), being the central element of the multimeric apoptosome. The apoptosome consists of cytochrome c, procaspase-9 and seven Apaf 1 monomers, and is considered to be core apoptotic machinery that executes mitochondria-dependent apoptosis. Cytochrome c, normally compartmentalized in the mitochorndria, is released into the cytoplasm following apoptotic stimuli. Apaf 1 binds cytochrome c in the cytoplasm and in the presence of dATP/ATP forms the apoptosome. The apoptosome binds procaspase-9 and promotes its autocatalytic activation. Active caspase-9, in turn, activates downstream caspases including 3, 6, and 7 contributing to the proteolytic caspase activation cascade which leads to cell death. Apaf 1 is an approx. 130 kDa protein. Alternative Apaf 1 splicing isoforms of Apaf 1 have been identified in mammalian cells, each having either or both an 11 amino acid insertion in the N-terminus and an additional WD40 repeat (43 aa) in the C-terminus; please refer to AceView of NCBI (http://www.ncbi.nih.gov/IEB/Research/Acembly/) for detailed information. Additionally, Apaf 1 can be proteolytically processed by active caspase-3 during apoptosis resulting in an Apaf 1 cleavage fragment of ~84 kDa (Lauber et al. 2001).

Long Name

Apoptotic Peptidase Activating Factor 1

Alternate Names

APAF1

Gene Symbol