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Recombinant Human Apolipoprotein A-I/ApoA1 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34869

Novus Biologicals, part of Bio-Techne
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NBP2-34869-100ug
NBP2-34869-1mg
NBP2-34869-500ug

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 243 amino acids corresponding to Apolipoprotein A-I/ApoA1 Source: E. coli

Uniprot ID: P02647

Amino Acid Sequence: DEPPQSPWDR VKDLATVYVD VLKDSGRDYV SQFEGSALGK QLNLKLLDNW DSVTSTFSKL REQLGPVTQE FWDNLEKETE GLRQEMSKDL EEVKAKVQPY LDDFQKKWQE EMELYRQKVE PLRAELQEGA RQKLHELQEK LSPLGEEMRD RARAHVDALR THLAPYSDEL RQRLAARLEA LKENGGARLA EYHAKATEHL STLSEKAKPA LEDLRQGLLP VLESFKVSFL SALEEYTKKL NTQ

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Apolipoprotein A-I/ApoA1 as determined by LAL method.

Predicted Molecular Mass

28.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Apolipoprotein A-I/ApoA1 Protein

SDS-PAGE: Recombinant Human Apolipoprotein A-I/ApoA1 Protein [NBP2-34869]

SDS-PAGE: Recombinant Human Apolipoprotein A-I/ApoA1 Protein [NBP2-34869]

SDS-Page: Recombinant Human Apolipoprotein A-I/ApoA1 Protein [NBP2-34869]

Formulation, Preparation and Storage

NBP2-34869
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Apolipoprotein A-I/ApoA1

Apolipoprotein A-I encoded by the APOA1 gene is the major protein component of high density lipoprotein (HDL) in plasma. It has a specific role in lipid metabolism. Apolipoprotein A-I participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). Apolipoprotein A-I is also isolated as a prostacyclin (PGI2) stabilizing factor and thus may have an anticlotting effect. Apolipoprotein A-I has been shown to interact with ABCA1, GPLD1 and PLTP. Apolipoprotein A-I binds to lipopolysaccharide or endotoxin and has a major role in the anti-endotoxin function of HDL. Mature human ApoA-1 shares 65 % and 62 % a.a. sequence identity with murine and rat ApoA-1, respectively. It has an approximate molecular weight of 28.1 kDa.

Alternate Names

Alp-1, APOA1, Apolipoprotein AI, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2

Gene Symbol

APOA1

UniProt

Additional Apolipoprotein A-I/ApoA1 Products

Product Documents for Recombinant Human Apolipoprotein A-I/ApoA1 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human Apolipoprotein A-I/ApoA1 Protein

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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