Recombinant Human ARC/NOL3 His Protein

Name: Recombinant Human ARC/NOL3 His Protein
Brand: Novus Biologicals
SKU: NBP2-23300

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23300

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Technical Data
Citations & Reviews

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all ARC Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-208 of Human ARC/NOL3

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGNAQER PSETIDRERK RLVETLQADS GLLLDALLAR GVLTGPEYEA LDALPDAERR VRRLLLLVQG KGEAACQELL RCAQRTAGAP DPAWDWQHVG PGYRDRSYDP PCPGHWTPEA PGSGTTCPGL PRASDPDEAG GPEGSEAVQS GTPEEPEPEL EAEASKEAEP EPEPEPELEP EAEAEPEPEL EPEPDPEPEP DFEERDESED S

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human ARC/NOL3 His Protein

SDS-PAGE: Recombinant Human ARC/NOL3 His Protein [NBP2-23300]

SDS-Page: Recombinant Human ARC/NOL3 His Protein [NBP2-23300] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP2-23300

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 10% glycerol, 1 mM DTT, 1 mM EDTA
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ARC

Nucleolar protein 3 (apoptosis repressor with CARD domain), also known as NOL3, is predominantly expressed in muscle and is present in the nucleoplasm and concentrated in nucleoli. NOL3 mRNA and protein levels are induced by neuronal activity, which is necessary to stimulate neuroplasticity, indicating a potential role for NOL3 in activity-dependent changes in dendrite function. NOL3 has been shown to localize to the cytoskeleton of neuronal cells and appears to colocalize with F-Actin, although it may associate with an actin-associated protein rather than directly with F-Actin. Recombinant human NOL3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Apoptosis Repressor with Caspase Recruitment Domain

Alternate Names

NOL3

Gene Symbol