Recombinant Human ARFIP1 His Protein

Name: Recombinant Human ARFIP1 His Protein
Brand: Novus Biologicals
SKU: NBP2-51834

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-51834

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Technical Data
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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all ARFIP1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-341 of Human ARFIP1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAQESPK NSAAEIPVTS NGEVDDSREH SFNRDLKHSL PSGLGLSETQ ITSHGFDNTK EGVIEAGAFQ GGQRTQTKSG PVILADEIKN PAMEKLELVR KWSLNTYKCT RQIISEKLGR GSRTVDLELE AQIDILRDNK KKYENILKLA QTLSTQLFQM VHTQRQLGDA FADLSLKSLE LHEEFGYNAD TQKLLAKNGE TLLGAINFFI ASVNTLVNKT IEDTLMTVKQ YESARIEYDA YRTDLEELNL GPRDANTLPK IEQSQHLFQA HKEKYDKMRN DVSVKLKFLE ENKVKVLHNQ LVLFHNAIAA YFAGNQKQLE QTLKQFHIKL KTPGVDAPSW LEEQ

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human ARFIP1 His Protein

SDS-PAGE: Recombinant Human ARFIP1 His Protein [NBP2-51834]

SDS-Page: ARFIP1 Recombinant Protein [NBP2-51834] - 15% SDS Page (3 ug)

Formulation, Preparation and Storage

NBP2-51834

Formulation	PBS (pH 7.4), 30% glycerol, 1 mM DTT
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ARFIP1

ADP-ribosylation factors, or ARFs, enhance the ADP ribosyltransferase activity of cholera toxin and are implicated in vesicle transport between endoplasmic reticulum and the Golgi complex. Arfaptin 1 is recruited from the cytosol to Golgi membranes by ARFs in a guanosine 5'-O-(3-thiotriphosphate)-dependent and brefeldin A-sensitive manner but is not a constituent of coatomer. Arfaptin 1 binds to nonmyristoylated GTP-bound ARF3, but not to GDP-bound ARF3, and also to ARF1, another class I ARF. It binds with lower affinity to ARF5, a class II ARF, and with very little affinity to ARF6, a class III ARF. POR1 (also designated arfaptin 2) was first isolated as a Rac 1 binding protein necessary for Rac mediated Actin polymerization and the subsequent formation of membrane ruffles and lamellipodia. POR1 has also been shown to interact with the ADP ribosylation factor ARF6, a GTPase that associates with the plasma membrane and intracellular endosome vesicles, in a GTP dependent manner. The association of POR1 with ARF6 stimulates induction of Actin polymerization. POR1 appears to play a regulatory role through multiple signaling pathways in the reorganization of the cytoskeletal structure.

Alternate Names

ADP-ribosylation factor interacting protein 1, ADP-ribosylation factor-interacting protein 1, arfaptin 1, arfaptin-1, HSU52521, MGC117369

Gene Symbol

ARFIP1

Additional ARFIP1 Products

Product Documents for Recombinant Human ARFIP1 His Protein

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COA

Product Specific Notices for Recombinant Human ARFIP1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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