Recombinant Human Arylsulfatase A/ARSA His Protein

Name: Recombinant Human Arylsulfatase A/ARSA His Protein
Brand: Novus Biologicals
SKU: NBP2-22695

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22695

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Arylsulfatase A/ARSA Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 21-509 of Human Arylsulfatase A/ARSA

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSRPPNIVL IFADDLGYGD LGCYGHPSST TPNLDQLAAG GLRFTDFYVP VSLCTPSRAA LLTGRLPVRM GMYPGVLVPS SRGGLPLEEV TVAEVLAARG YLTGMAGKWH LGVGPEGAFL PPHQGFHRFL GIPYSHDQGP CQNLTCFPPA TPCDGGCDQG LVPIPLLANL SVEAQPPWLP GLEARYMAFA HDLMADAQRQ DRPFFLYYAS HHTHYPQFSG QSFAERSGRG PFGDSLMELD AAVGTLMTAI GDLGLLEETL VIFTADNGPE TMRMSRGGCS GLLRCGKGTT YEGGVREPAL AFWPGHIAPG VTHELASSLD LLPTLAALAG APLPNVTLDG FDLSPLLLGT GKSPRQSLFF YPSYPDEVRG VFAVRTGKYK AHFFTQGSAH SDTTADPACH ASSSLTAHEP PLLYDLSKDP GENYNLLGGV AGATPEVLQA LKQLQLLKAQ LDAAVTFGPS QVARGEDPAL QICCHPGCTP RPACCHCPDP HA

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

54.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Arylsulfatase A/ARSA His Protein

SDS-PAGE: Recombinant Human Arylsulfatase A/ARSA His Protein [NBP2-22695]

SDS-Page: Recombinant Human Arylsulfatase A/ARSA Protein [NBP2-22695]

Formulation, Preparation and Storage

NBP2-22695

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Arylsulfatase A/ARSA

ARSA hydrolyzes cerebrosidesulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene. Recombinant human ARSA protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

ARSA, Cerebroside 3-sulfatase

Gene Symbol

ARSA

Additional Arylsulfatase A/ARSA Products

Product Documents for Recombinant Human Arylsulfatase A/ARSA His Protein

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COA

Product Specific Notices for Recombinant Human Arylsulfatase A/ARSA His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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