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Recombinant Human ASMT His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22666

Novus Biologicals, part of Bio-Techne
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NBP2-22666

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-298 of Human ASMT

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSSEDQAYR LLNDYANGFM VSQVLFAACE LGVFDLLAEA PGPLDVAAVA AGVRASAHGT ELLLDICVSL KLLKVETRGG KAFYRNTELS SDYLTTVSPT SQCSMLKYMG RTSYRCWGHL ADAVREGRNQ YLETFGVPAE ELFTAIYRSE GERLQFMQAL QEVWSVNGRS VLTAFDLSVF PLMCDLGGDF FKDPLPEADL YILARVLHDW ADGKCSHLLE RIYHTCKPGG GILVIESLLD EDRRGPLLTQ LYSLNMLVQT EGQERTPTHY HMLLSSAGFR DFQFKKTGAI YDAILARK

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

35.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human ASMT His Protein

SDS-PAGE: Recombinant Human ASMT His Protein [NBP2-22666]

SDS-PAGE: Recombinant Human ASMT His Protein [NBP2-22666]

SDS-Page: Recombinant Human ASMT His Protein [NBP2-22666] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP2-22666
Formulation 20 mM Tris-HCl buffer (pH 8.0), 1 M UREA, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ASMT

ASMT belongs to the methyltransferase superfamily and is involved in melatonin biosynthesis. Expressed in brain, retina and pineal gland, ASMT functions to catalyze the final reaction in the synthesis of melatonin, specifically the conversion of S-adenosyl-L-methionine and N-acetylserotonin to S-adenosyl-L-homocysteine and melatonin. Recombinant human ASMT protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

acetylserotonin N-methyltransferase, acetylserotonin O-methyltransferaseEC 2.1.1.4, ASMTY, HIOMTacetylserotonin methyltransferase (Y chromosome), HIOMTY, hydroxyindole O-methyltransferase

Gene Symbol

ASMT

Additional ASMT Products

Product Documents for Recombinant Human ASMT His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human ASMT His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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