Recombinant Human ATG10 His Protein

Name: Recombinant Human ATG10 His Protein
Brand: Novus Biologicals
SKU: NBP2-22702

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22702

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Technical Data
Citations & Reviews

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

Q9H0Y0

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all ATG10 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-220 of Human ATG10

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMEEDEFI GEKTFQRYCA EFIKHSQQIG DSWEWRPSKD CSDGYMCKIH FQIKNGSVMS HLGASTHGQT CLPMEEAFEL PLDDCEVIET AAASEVIKYE YHVLYSCSYQ VPVLYFRASF LDGRPLTLKD IWEGVHECYK MRLLQGPWDT ITQQEHPILG QPFFVLHPCK TNEFMTPVLK NSQKINKNVN YITSWLSIVG PVVGLNLPLS YAKATSQDER NVP

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

27.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human ATG10 His Protein

SDS-PAGE: Recombinant Human ATG10 His Protein [NBP2-22702]

SDS-Page: Atg10 Protein [NBP2-22702]

Formulation, Preparation and Storage

NBP2-22702

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 10% glycerol, 1 mM DTT
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATG10

Ubiquitin-like-conjugating enzyme ATG10, also known as ATG10, is a 220 amino acid protein that localizes to the cytoplasm and plays a role in autophagy, specifically functioning as an E2-like enzyme that provides Atg recognition sites during autophagosome synthesis. ATG10 has also been shown to interact with ATG12 in human embryonic kidney cells in the presence of ATG7. Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome, while deletion of the q arm or of chromosome 5 altogether is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome. Recombinant human ATG10 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Autophagy Related 10 Homolog

Alternate Names

APG10, APG10L, PP12616

Gene Symbol

ATG10

UniProt