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Recombinant Human ATP5H His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22700

Novus Biologicals, part of Bio-Techne
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NBP2-22700

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-161 of Human ATP5H

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAGRKLA LKTIDWVAFA EIIPQNQKAI ASSLKSWNET LTSRLAALPE NPPAIDWAYY KANVAKAGLV DDFEKKFNAL KVPVPEDKYT AQVDAEEKED VKSCAEWVSL SKARIVEYEK EMEKMKNLIP FDQMTIEDLN EAFPETKLDK KKYPYWPHQP IENL

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

20.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human ATP5H His Protein

SDS-PAGE: Recombinant Human ATP5H His Protein [NBP2-22700]

SDS-PAGE: Recombinant Human ATP5H His Protein [NBP2-22700]

SDS-Page: ATP5H Protein [NBP2-22700]

Formulation, Preparation and Storage

NBP2-22700
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATP5H

ATP synthase subunit d, also known as ATP5H, is a 161 amino acid protein that belongs to the ATPase d subunit family. ATP5H encodes the d subunit of the F0 complex. ATP5H produces ATP from ADP in the presence of a proton gradient across the membrane, which is generated by electron transport complexes of the respiratory chain. Localizing to mitochondrial inner membrane, ATP5H exists as two alternatively spliced isoforms and is encoded by a gene that maps to human chromosome 17q25.1. Recombinant human ATP5H protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

ATP synthase D chain, mitochondrial, ATP synthase subunit d, mitochondrial, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d, ATP synthase, H+ transporting, mitochondrial F1F0, subunit d, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d, ATP5JD, ATPase subunit d, ATPQ, My032 protein

Gene Symbol

ATP5PD

Additional ATP5H Products

Product Documents for Recombinant Human ATP5H His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human ATP5H His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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