Recombinant Human ATP6V0E1 GST (N-Term) Protein

Name: Recombinant Human ATP6V0E1 GST (N-Term) Protein
Brand: Novus Biologicals
SKU: H00008992-P01

Novus Biologicals, part of Bio-Techne | Catalog # H00008992-P01

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

Wheat germ

Tag

GST (N-Term)

Conjugate

Unconjugated

Applications

ELISA, Affinity Purification, Microarray, Western Blot

View all ATP6V0E1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with GST tag at N-terminal corresponding to the amino acids 29587 of Human ATP6V0E

Source: Wheat Germ (in vitro)

Amino Acid Sequence: MAYHGLTVPLIVMSVFWGFVGFLVPWFIPKGPNRGVIITMLVTCSVCCYLFWLIAILAQLNPLFGPQLKNETIWYLKYHWP

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

35.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human ATP6V0E1 GST (N-Term) Protein

SDS-PAGE: Recombinant Human ATP6V0E1 GST (N-Term) Protein [H00008992-P01]

SDS-Page: ATP6V0E1 Recombinant Protein [H00008992-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Formulation, Preparation and Storage

H00008992-P01

Preparation Method	in vitro wheat germ expression system
Formulation	50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative	No Preservative
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -80C. Avoid freeze-thaw cycles.

Background: ATP6V0E1

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is possibly part of the V0 subunit. Since two nontranscribed pseudogenes have been found in dog, it is possible that the localization to chromosome 2 for this gene by radiation hybrid mapping is representing a pseudogene. Genomic mapping puts the chromosomal location on 5q35.3.

Alternate Names

ATP6HVma21, ATP6V0, ATP6V0EVma21p, ATPase, H+ transporting, lysosomal (vacuolar proton pump) 9kD, ATPase, H+ transporting, lysosomal 9kDa, V0 subunit e, ATPase, H+ transporting, lysosomal 9kDa, V0 subunit e1, H(+)-transporting two-sector ATPase, subunit H, M9.2, vacuolar ATP synthase subunit H, vacuolar proton pump H subunit, Vacuolar proton pump subunit e 1, vacuolar proton-ATPase subunit M9.2, V-ATPase 9.2 kDa membrane accessory protein, V-ATPase H subunit, V-ATPase M9.2 subunit, V-ATPase subunit e 1, V-type proton ATPase subunit e 1

Gene Symbol

ATP6V0E1

Additional ATP6V0E1 Products

Product Documents for Recombinant Human ATP6V0E1 GST (N-Term) Protein

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COA

SDS

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Product Specific Notices for Recombinant Human ATP6V0E1 GST (N-Term) Protein

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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