Recombinant Human Beclin 1 His Protein

Name: Recombinant Human Beclin 1 His Protein
Brand: Novus Biologicals
SKU: NBP2-22710

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22710

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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Beclin 1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-450 of Human Beclin 1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MEGSKTSNNS TMQVSFVCQR CSQPLKLDTS FKILDRVTIQ ELTAPLLTTA QAKPGETQEE ETNSGEEPFI ETPRQDGVSR RFIPPARMMS TESANSFTLI GEASDGGTME NLSRRLKVTG DLFDIMSGQT DVDHPLCEEC TDTLLDQLDT QLNVTENECQ NYKRCLEILE QMNEDDSEQL QMELKELALE EERLIQELED VEKNRKIVAE NLEKVQAEAE RLDQEEAQYQ REYSEFKRQQ LELDDELKSV ENQMRYAQTQ LDKLKKTNVF NATFHIWHSG QFGTINNFRL GRLPSVPVEW NEINAAWGQT VLLLHALANK MGLKFQRYRL VPYGNHSYLE SLTDKSKELP LYCSGGLRFF WDNKFDHAMV AFLDCVQQFK EEVEKGETRF CLPYRMDVEK GKIEDTGGSG GSYSIKTQFN SEEQWTKALK FMLTNLKWGL AWVSSQFYNK

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

54.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Beclin 1 His Protein

SDS-PAGE: Recombinant Human Beclin 1 His Protein [NBP2-22710]

SDS-Page: Recombinant Human Beclin 1 Protein [NBP2-22710] -

Formulation, Preparation and Storage

NBP2-22710

Formulation	20 mM Tris-HCl buffer (pH 8.5), 0.1 M NaCl, 10% glycerol, 1 mM DTT
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Beclin 1

BECN1, also as known as Beclin-1 and autophagy-related gene (Atg) 6, belongs to the beclin family. This protein plays a central role in autophagy. Beclin-1 and its binding partner class III phosphoinositide 3-kinase (PI3K), also named Vps34, are required for the initiation of the formation of the autophagasome in autophagy. Also it required for the abcission step of cytokinesis. BECN1 plays a role in antiviral host defense. Recombinant human BECN1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Long Name

Beclin1, Autophagy-related

Alternate Names

ATG6, BECN1, GT197, VPS30

Gene Symbol

BECN1

Additional Beclin 1 Products

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COA

Product Specific Notices for Recombinant Human Beclin 1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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