Recombinant Human beta B1 Crystallin His Protein

Name: Recombinant Human beta B1 Crystallin His Protein
Brand: Novus Biologicals
SKU: NBP1-44470

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44470

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all beta B1 Crystallin Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-252 of Human beta B1 Crystallin

Source: E.coli

Amino Acid Sequence: MSQAAKASAS ATVAVNPGPD TKGKGAPPAG TSPSPGTTLA PTTVPITSAK AAELPPGNYR LVVFELENFQ GRRAEFSGEC SNLADRGFDR VRSIIVSAGP WVAFEQSNFR GEMFILEKGE YPRWNTWSSS YRSDRLMSFR PIKMDAQEHK ISLFEGANFK GNTIEIQGDD APSLWVYGFS DRVGSVKVSS GTWVGYQYPG YRGYQYLLEP GDFRHWNEWG AFQPQMQSLR RLRDKQWHLE GSFPVLATEP PKRSHHHHHH

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

29.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human beta B1 Crystallin His Protein

SDS-PAGE: Recombinant Human beta B1 Crystallin His Protein [NBP1-44470]

SDS-Page: beta B1 Crystallin Protein [NBP1-44470] - CRYBB1, 29.1 kDa (260aa) confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Formulation, Preparation and Storage

NBP1-44470

Formulation	20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1 mM DTT
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: beta B1 Crystallin

Crystallins are the major structural proteins of the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. Crystallins are divided into alpha, beta, and gamma families. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. CRYBB1 is a beta basic group member and undergoes extensive cleavage at its N-terminal extension during lens maturation. Recombinant human CRYBB1 protein, fused to His-tag at C-terminus was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

Beta-B1 crystallin, beta-crystallin B1, CATCN3, crystallin, beta B1, eye lens structural protein

Gene Symbol

CRYBB1

Additional beta B1 Crystallin Products

Product Documents for Recombinant Human beta B1 Crystallin His Protein

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COA

Product Specific Notices for Recombinant Human beta B1 Crystallin His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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