Recombinant Mouse beta-Defensin 3 Protein

Name: Recombinant Mouse beta-Defensin 3 Protein
Brand: Novus Biologicals
SKU: NBP2-35146

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35146

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

Q9WTL0

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all beta-Defensin 3 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 41 amino acid residues corresponding to beta-Defensin 3 Source: E. coli

Uniprot ID: Q9WTL0

Amino Acid Sequence: KKINNPVSCL RKGGRCWNRC IGNTRQIGSC GVPFLKCCKR K

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of beta-Defensin 3 as determined by LAL method.

Predicted Molecular Mass

4.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Defensin beta 3 Protein is fully biologically active when compared to standard. The ED50 as determined by anti-microbial activity against E.coli. is less than 20 ug/ml, corresponding to a specific activity of > 50 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse beta-Defensin 3 Protein

SDS-PAGE: Recombinant Mouse beta-Defensin 3 Protein [NBP2-35146]

SDS-Page: Mouse beta-Defensin 3 Protein [NBP2-35146]

Formulation, Preparation and Storage

NBP2-35146

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: beta-Defensin 3

Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6 k Da proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. beta-Defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Four human beta-defensins have been identified and they are expressed on some leukocytes and at epithelial surfaces. Because beta-defensins is cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca 2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane.

Alternate Names

BD14, BD3, betaDefensin 3, DEFB103A, DEFB103B, DEFB3, HBD-3

Gene Symbol

DEFB103B

UniProt