Recombinant E. coli beta-lactamase Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61444
Key Product Details
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 264 amino acids corresponding to beta-lactamase Source: E. coli
Amino Acid Sequence: MHPETLVKVK DAEDQLGARV GYIELDLNSG KILESFRPEE RFPMMSTFKV LLCGAVLSRV DAGQEQLGRR IHYSQNDLVE YSPVTEKHLT DGMTVRELCS AAITMSDNTA ANLLLTTIGG PKELTAFLHN MGDHVTRLDR WEPELNEAIP NDERDTTMPA AMATTLRKLL TGELLTLASR QQLIDWMEAD KVAGPLLRSA LPAGWFIADK SGAGERGSRG IIAALGPDGK PSRIVVIYTT GSQATMDERN RQIAEIGASL IKHW
Purity
>95%, by SDS-PAGE
Predicted Molecular Mass
28.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Fully biologically active when compared to standard. One unit of enzyme activity is defined as the amount of enzyme which will hydrolyze 1.0 umol of benzyl penicillin in presence of EDTA at pH 7.0 and at 25 C.
Protein / Peptide Type
Recombinant Protein
Formulation, Preparation and Storage
NBP2-61444
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in 100 mM Tris, pH 7.0. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: beta-lactamase
Alternate Names
ampA, AmpC, cephalosporinase, EC 3.5.2.6, ECK4146
Gene Symbol
ampC
UniProt
Additional beta-lactamase Products
Product Documents for Recombinant E. coli beta-lactamase Protein
Product Specific Notices for Recombinant E. coli beta-lactamase Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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