Can you protein fluoresence labeled BMP2 protein?

Name: Recombinant Human BMP-2 Protein
Brand: Novus Biologicals
SKU: NBP2-34928

Unfortunately, we don't custom make any fluorophore conjugation to protein, but only making fluorophore-antibody conjugation in house. This is because proteins diverse a lot, with the different number of sites that can be conjugated with fluorophores and thus the efficiency and the successful rate cannot be determined.

Recombinant Human BMP-2 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34928

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P12643

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all BMP-2 Proteins and Enzymes »

Product Specifications

Description

A homodimeric protein consisting of two 115 amino acid non-glycosylated polypeptide chains corresponding to BMP-2 Source: E. coli

Uniprot ID: P12643

Amino Acid Sequence: MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of BMP-2 as determined by LAL method.

Predicted Molecular Mass

26.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

BMP2 protein is fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 200 ng/ml, corresponding to a specific activity of > 5.0 x 10^3 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human BMP-2 Protein

SDS-PAGE: Recombinant Human BMP-2 Protein [NBP2-34928]

SDS-Page: Human BMP-2 Protein [NBP2-34928] - The Recombinant Human BMP-2 homodimeric protein is resolved by SDS-PAGE under reducing (R) and non-reducing (NR) conditions.

Formulation, Preparation and Storage

NBP2-34928

Formulation	Lyophilized from a 0.2 um filtered concentrated solution containing 10 mM sodium citrate pH 3.5.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled H2O to a concentration of 0.1-1.0mg/mL. Do not reconstitute in PBS or cell culture media.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: BMP-2

Bone morphogenetic protein 2 plays a dominant role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration. Mature BMP-2 forms disulfide linked homodimers and heterodimers with BMP-7. BMP-2 induces cartilage and bone formation, and BMP-2/BMP-7 heterodimers are even more potent than BMP-2 homodimers. BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine production, and is required for cardiac contractility.

Long Name

Bone Morphogenetic Protein 2

Alternate Names

BDA2, BMP2, SSFSC

Gene Symbol

BMP2

UniProt