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Recombinant Human BMP-3 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61322

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP2-61322-100ug
NBP2-61322-10ug
NBP2-61322-1mg
NBP2-61322-500ug

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Description

A homodimeric protein consisting of two 110 amino acid non-glycosylated polypeptide chains corresponding to BMP-3 Source: E. coli

Amino Acid Sequence: QWIEPRNCAR RYLKVDFADI GWSEWIISPK SFDAYYCSGA CQFPMPKSLK PSNHATIQSI VRAVGVVPGI PEPCCVPEKM SSLSILFFDE NKNVVLKVYP NMTVESCACR

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of BMP-3 as determined by LAL method.

Predicted Molecular Mass

24.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

BMP-3 protein is fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit BMP-2-induced activity in murine MC3T3-E1 cells.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-61322
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: BMP-3

Bone morphogenetic protein 3 (BMP3) is a 472 amino acid member of the bone morphogenetic protein (BMP) family. BMP3 negatively regulates bone density and is a member of the TGF beta superfamily. It is highly expressed in fracture tissue, predominantly in osteoblasts, osteoclasts and chondroblasts. It may play a role in otosclerosis, osteosarcoma, osteogenesis imperfecta, osteomalacia and periodontitis.

Long Name

Bone Morphogenetic Protein 3

Alternate Names

BMP3, Osteogenin

Gene Symbol

BMP3

Additional BMP-3 Products

Product Documents for Recombinant Human BMP-3 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human BMP-3 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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