Recombinant Human BRDG1/STAP-1 His Protein

Name: Recombinant Human BRDG1/STAP-1 His Protein
Brand: Novus Biologicals
SKU: NBP1-98975

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-98975

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Technical Data
Citations & Reviews

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all BRDG1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-295 of Human BRDG1/STAP-1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMMAKKP PKPAPRRIFQ ERLKITALPL YFEGFLLIKR SGYREYEHYW TELRGTTLFF YTDKKSIIYV DKLDIVDLTC LTEQNSTEKN CAKFTLVLPK EEVQLKTENT ESGEEWRGFI LTVTELSVPQ NVSLLPGQVI KLHEVLEREK KRRIETEQST SVEKEKEPTE DYVDVLNPMP ACFYTVSRKE ATEMLQKNPS LGNMILRPGS DSRNYSITIR QEIDIPRIKH YKVMSVGQNY TIELEKPVTL PNLFSVIDYF VKETRGNLRP FICSTDENTG QEPSMEGRSE KLKKNPHIA

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

36.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human BRDG1/STAP-1 His Protein

SDS-PAGE: Recombinant Human BRDG1/STAP-1 His Protein [NBP1-98975]

SDS-Page: Recombinant Human BRDG1/STAP-1 Protein [NBP1-98975] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-98975

Formulation	20 mM Tris-HCl buffer (pH8.0), 30% glycerol, 0.1 M NaCl, 1 mM DTT
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BRDG1

STAP1, also known as signal-transducing adaptor protein 1, appears to functions as a docking protein acting downstream of Tec tyrosine kinase in B cell antigen receptor signaling. The protein is directly phosphorylated by Tec in vitro where it participates in a positive feedback loop, increasing Tec activity. Recombinant human STAP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Long Name

BCR Downstream-signaling Protein 1

Alternate Names

STAP1

Gene Symbol