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Recombinant E. coli Carbonic Anhydrase II/CA2 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52240

Novus Biologicals, part of Bio-Techne
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NBP2-52240-0.02mg
NBP2-52240-0.1mg

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-220 of E.coli Carbonic Anhydrase II/CA2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MKDIDTLISN NALWSKMLVE EDPGFFEKLA QAQKPRFLWI GCSDSRVPAE RLTGLEPGEL FVHRNVANLV IHTDLNCLSV VQYAVDVLEV EHIIICGHYG CGGVQAAVEN PELGLINNWL LHIRDIWFKH SSLLGEMPQE RRLDTLCELN VMEQVYNLGH STIMQSAWKR GQKVTIHGWA YGIHDGLLRD LDVTATNRET LEQRYRHGIS NLKLKHANHK

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

27.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 1,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli Carbonic Anhydrase II/CA2 His Protein

SDS-PAGE: Recombinant E. coli Carbonic Anhydrase II/CA2 His Protein [NBP2-52240]

SDS-PAGE: Recombinant E. coli Carbonic Anhydrase II/CA2 His Protein [NBP2-52240]

SDS-Page: Recombinant E. coli Carbonic Anhydrase II/CA2 Protein [NBP2-52240] - 15% SDS Page (3 ug)

Formulation, Preparation and Storage

NBP2-52240
Formulation 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase II/CA2

Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20

Alternate Names

CA2

Gene Symbol

CA2

Additional Carbonic Anhydrase II/CA2 Products

Product Documents for Recombinant E. coli Carbonic Anhydrase II/CA2 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant E. coli Carbonic Anhydrase II/CA2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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