Recombinant Human Cathepsin F His Protein

Name: Recombinant Human Cathepsin F His Protein
Brand: Novus Biologicals
SKU: NBP3-11848

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11848

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Key Product Details

Source

Baculovirus

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

View all Cathepsin F Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a His-tag and corresponding to the amino acids 20-484 of Human Cathepsin F.

Source: Baculovirus

Amino Acid Sequence: ADL APAQPRA ASFQAWGPPS PELLAPTRFA LEMFNRGRAA GTRAVLGLVR GRVRRAGQGS LYSLEATLEE PPCNDPMVCR LPVSKKTLLC SFQVLDELGR HVLLRKDCGP VDTKVPGAGE PKSAFTQGSA MISSLSQNHP DNRNETFSSV ISLLNEDPLS QDLPVKMASI FKNFVITYNR TYESKEEARW RLSVFVNNMV RAQKIQALDR GTAQYGVTKF SDLTEEEFRT IYLNTLLRKE PGNKMKQAKS VGDLAPPEWD WRSKGAVTKV KDQGMCGSCW AFSVTGNVEG QWFLNQGTLL SLSEQELLDC DKMDKACMGG LPSNAYSAIK NLGGLETEDD YSYQGHMQSC NFSAEKAKVY INDSVELSQN EQKLAAWLAK RGPISVAINA FGMQFYRHGI SRPLRPLCSP WLIDHAVLLV GYGNRSDVPF WAIKNSWGTD WGEKGYYYLH RGSGACGVNT MASSAVVD

Purity

>90%, by SDS-PAGE

Endotoxin Level

< 1 EU per 1ug of protein (determined by LAL method)

Predicted Molecular Mass

52.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 5 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1pmole of Z-Phe-Arg-AMC to Z-Phe-Arg and AMC per minute at pH 5.0 at 37.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Cathepsin F His Protein

SDS-PAGE: Recombinant Human Cathepsin F His Protein [NBP3-11848]

SDS-Page: Recombinant Human Cathepsin F His Protein [NBP3-11848] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Formulation, Preparation and Storage

NBP3-11848

Formulation	PBS (pH 7.4), 40% glycerol
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cathepsin F

Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system. Cathepsins generally contain a signal sequence, followed by a propeptide and then a catalytically active mature region. The very long (251 amino acid residues) proregion of the cathepsin F precursor contains a C-terminal domain similar to the pro-segment of cathepsin L-like enzymes, a 50-residue flexible linker peptide, and an N-terminal domain predicted to adopt a cystatin-like fold. The cathepsin F proregion is unique within the papain family cysteine proteases in that it contains this additional N-terminal segment predicted to share structural similarities with cysteine protease inhibitors of the cystatin superfamily. This cystatin-like domain contains some of the elements known to be important for inhibitory activity. CTSF encodes a predicted protein of 484 amino acids which contains a 19 residue signal peptide. Cathepsin F contains five potential N-glycosylation sites, and it may be targeted to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway. The cathepsin F gene is ubiquitously expressed, and it maps to chromosome 11q13, close to the gene encoding cathepsin W.

Alternate Names

CTSF

Gene Symbol