Recombinant Human CCL1/I-309/TCA-3 Protein

Name: Recombinant Human CCL1/I-309/TCA-3 Protein
Brand: Novus Biologicals
SKU: NBP2-35886

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35886

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P22362

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CCL1/I-309/TCA-3 Proteins and Enzymes »

Product Specifications

Description

A single, non-glycosylated polypeptide chain containing 74 amino acids corresponding to CCL1/I-309/TCA-3 Source: E. coli

Uniprot ID: P22362

Amino Acid Sequence: SKSMQVPFSR CCFSFAEQEI PLRAILCYRN TSSICSNEGL IFKLKRGKEA CALDTVGWVQ RHRKMLRHCP SKRK

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL1/I-309/TCA-3 as determined by LAL method.

Predicted Molecular Mass

8.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CCL1/I-309/TCA-3 Protein

SDS-PAGE: Recombinant Human CCL1/I-309/TCA-3 Protein [NBP2-35886]

SDS-Page: Human CCL1/I-309/TCA-3 Protein [NBP2-35886]

Formulation, Preparation and Storage

NBP2-35886

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL1/I-309/TCA-3

Chemokine (C-C motif) ligand 1 (CCL1) belongs to a family inflammatory cytokines and also known as chemokines. It is a small glycoprotein secreted by activated T cells with a molecular weight of approximately 8.5 kDa. CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42 % amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines.

Alternate Names

I-309, P500, SCYA1, SCYA2, TCA-3, TCA3, CCL1, I-309/CCL1

Gene Symbol

CCL1

UniProt