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Recombinant Rhesus Macaque CCL11/Eotaxin Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35282

Novus Biologicals, part of Bio-Techne
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NBP2-35282-100ug
NBP2-35282-1mg
NBP2-35282-500ug
NBP2-35282-5ug

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 74 amino acids corresponding to CCL11/Eotaxin Source: E. coli

Uniprot ID: Q8MIT7

Amino Acid Sequence: GPDSVATTCC FTLTNKKIPL QRLESYRRII SGKCPQKAVI FKTKLAKDIC ADPKKKWVQD SMKYLDRKSP TPKP

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of CCL11/Eotaxin as determined by LAL method.

Predicted Molecular Mass

8.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CCL11/Eotaxin protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration range of 0.1-10.0 ng/ml

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35282
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 6.9.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL11/Eotaxin

CCL11 is belonging to the CC chemokine family. It is encoded by the gene CCL11. CCL11 was first purified from bronchoalveolar lavage fluid of guinea pigs. It was a strong and specific eosinophil chemoattractant in vitro. It can directly chemotactic for eosinophils, but not for monocytes or neutrophils. Recombinant Rhesus Macaque CCL11 is a 74 amino acid (a.a.) protein and it shares 89%, and 57%, and 60% a.a. sequence identity with human, murine and rat CCL11.

Alternate Names

Eotaxin, CCL11, Eotaxin/CCL11

Gene Symbol

CCL11

Additional CCL11/Eotaxin Products

Product Documents for Recombinant Rhesus Macaque CCL11/Eotaxin Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Rhesus Macaque CCL11/Eotaxin Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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