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Recombinant Human CCL15/MIP-1 delta Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35043

Novus Biologicals, part of Bio-Techne
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NBP2-35043-100ug
NBP2-35043-1mg
NBP2-35043-500ug
NBP2-35043-5ug

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 68 amino acids corresponding to CCL15/MIP-1 delta Source: E. coli

Uniprot ID: Q16663

Amino Acid Sequence: SFHFAADCCT SYISQSIPCS LMKSYFETSS ECSKPGVIFL TKKGRQVCAK PSGPGVQDCM KKLKPYSI

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of CCL15/MIP-1 delta as determined by LAL method.

Predicted Molecular Mass

7.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CCL15/MIP-1 delta protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CCL15/MIP-1 delta Protein

SDS-PAGE: Recombinant Human CCL15/MIP-1 delta Protein [NBP2-35043]

SDS-PAGE: Recombinant Human CCL15/MIP-1 delta Protein [NBP2-35043]

SDS-Page: Human CCL15/MIP-1 delta 68 a.a Protein [NBP2-35043]

Formulation, Preparation and Storage

NBP2-35043
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 1 x PBS, pH 7.2.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL15/MIP-1 delta

Human CCL15 is belonging to the CC chemokine family and shares 35 % amino acid homology with human HCC1 (CCL14). CCL15 is most abundant in heart, skeletal muscle and adrenal gland, and low expressed in liver, small intestine, colon, and in certain leukocytes and macrophages of the lung. It is chemotactic for neutrophils, monocytes, and lymphocytes and elicits its effects by binding to cell surface chemokine receptors like CCR1 and CCR3. CCL15 has several cleaved chains. All of them are more potent chemoattractants than CCL15.

Alternate Names

HCC-2, Leukotactin 1, MIP-1 delta, MIP-5, MIP1 delta, NCC-3, CCL15, MIP-5/CCL15

Gene Symbol

CCL15

UniProt

Additional CCL15/MIP-1 delta Products

Product Documents for Recombinant Human CCL15/MIP-1 delta Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human CCL15/MIP-1 delta Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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