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Recombinant Mouse CCL19/MIP-3 beta Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35095

Novus Biologicals, part of Bio-Techne
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NBP2-35095-100ug
NBP2-35095-1mg
NBP2-35095-500ug
NBP2-35095-5ug

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 83 amino acids corresponding to CCL19/MIP-3 beta Source: E. coli

Uniprot ID: O70460

Amino Acid Sequence: GANDAEDCCL SVTQRPIPGN IVKAFRYLLN EDGCRVPAVV FTTLRGYQLC APPDQPWVDR IIRRLKKSSA KNKGNSTRRS PVS

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL19/MIP-3 beta as determined by LAL method.

Predicted Molecular Mass

9.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Macrophage Inflammatory Protein 3 beta Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human mature dendritic cells is in a concentration range of 10-100 ng/ml.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35095
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL19/MIP-3 beta

CCL19, also known as EBI1 Ligands chemokine (ELC) and macrophage inflammatory protein-3-beta, is encoded by the CCL19 gene which is located on chromosome 4 in mouse. The protein is expressed at high levels in thymus and lymph nodes, with intermediate levels in trachea and colon and low levels in stomach, lung and some other organs. CCL19 is signal through CCR7 and chemotaxis of T and B lymphocytes and myeloid progenitor cells.

Alternate Names

ELC, MIP-3 beta, MIP3 beta, CCL19, MIP-3 beta/CCL19

Gene Symbol

CCL19

UniProt

Additional CCL19/MIP-3 beta Products

Product Documents for Recombinant Mouse CCL19/MIP-3 beta Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Mouse CCL19/MIP-3 beta Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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