Recombinant Mouse CCL20/MIP-3 alpha Protein

Name: Recombinant Mouse CCL20/MIP-3 alpha Protein
Brand: Novus Biologicals
SKU: NBP2-35091

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35091

Submit a review

Product Datasheet / COA

Catalog #

Availability

Size / Price

Qty

100% Guarantee

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

O89093

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CCL20/MIP-3 alpha Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 70 amino acids corresponding to CCL20/MIP-3 alpha Source: E. coli

Uniprot ID: O89093

Amino Acid Sequence: ASNYDCCLSY IQTPLPSRAI VGFTRQMADE ACDINAIIFH TKKRKSVCAD PKQNWVKRAV NLLSLRVKKM

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL20/MIP-3 alpha as determined by LAL method.

Predicted Molecular Mass

8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CCL20/MIP-3 alpha protein is fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human CCR6 transfected murine BaF3 cells is in a concentration range of 0.1-10 ng/ml

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse CCL20/MIP-3 alpha Protein

SDS-PAGE: Recombinant Mouse CCL20/MIP-3 alpha Protein [NBP2-35091]

SDS-Page: Mouse CCL20/MIP-3 alpha Protein [NBP2-35091]

Formulation, Preparation and Storage

NBP2-35091

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL20/MIP-3 alpha

CCL20, also named LARC (Liver and Activation-regulated Chemokine), is encoded by CCL20 located on chromosome 1 in mouse. The expression can be induced by LPS and some inflammatory cytokines, and it is at a high level in lymph nodes, PBL, fatal liver, fatal lung, appendix and so on. CCL20 has functions of chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors. CCL20 elicits it effects by binding with CCR6 receptor.

Alternate Names

exodus-1, LARC, MIP-3 alpha, MIP3 alpha, CCL20, MIP-3, MIP-3 alpha/CCL20

Gene Symbol

CCL20

UniProt