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Recombinant Mouse CCL21/6Ckine Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35179

Novus Biologicals, part of Bio-Techne
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NBP2-35179-100ug
NBP2-35179-1mg
NBP2-35179-500ug
NBP2-35179-5ug

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

Product Specifications

Description

A single, non-glycosylated polypeptide chain containing 110 amino acids corresponding to CCL21/6Ckine Source: E. coli

Uniprot ID: P86792

Amino Acid Sequence: SDGGGQDCCL KYSQKKIPYS IVRGYRKQEP SLGCPIPAIL FLPRKHSKPE LCANPEEGWV QNLMRRLDQP PAPGKQSPGC RKNRGTSKSG KKGKGSKGCK RTEQTQPSRG

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL21/6Ckine as determined by LAL method.

Predicted Molecular Mass

12.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CCL21 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using murine T-lymphocytes is in a concentration range of 10-100 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse CCL21/6Ckine Protein

SDS-PAGE: Recombinant Mouse CCL21/6Ckine Protein [NBP2-35179]

SDS-PAGE: Recombinant Mouse CCL21/6Ckine Protein [NBP2-35179]

SDS-Page: Mouse CCL21/6Ckine Protein [NBP2-35179]

Formulation, Preparation and Storage

NBP2-35179
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL21/6Ckine

CCL21 is encoded by CCL21 gene that is located in Chr. 4 and shows 21-33 % identity to other CC chemokines. This chemokine contains the four conserved cysteines characteristic of beta chemokines plus two additional cysteines in its unusually long carboxylterminal domain. Human and mouse CCL21 are highly conserved, exhibiting 86 % amino acid sequence identity. CCL21 is highly expressed in lymph nodes of certain endothelial cells, and in the spleen and appendix. It elicits effects by binding with CCR7, having functions of T and B lymphocytes chemotaxis and inhibiting hematopoiesis.

Alternate Names

6Ckine, exodus-2, SCYA21, SLC, TCA-4, Exodus-2/CCL21

Gene Symbol

CCL21

Additional CCL21/6Ckine Products

Product Documents for Recombinant Mouse CCL21/6Ckine Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Mouse CCL21/6Ckine Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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