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Recombinant Rat CCL24/Eotaxin-2/MPIF-2 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35295

Novus Biologicals, part of Bio-Techne
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NBP2-35295-100ug
NBP2-35295-1mg
NBP2-35295-500ug
NBP2-35295-5ug

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

Product Specifications

Description

A single non-glycosylated polypeptide chain consisting of an N-terminal Methionine and the mature rat CCL24/Eotaxin-2/MPIF-2 Source: E. coli

Amino Acid Sequence: MPTGSVTIPS SCCVTFISKK IPVNRVISYQ LANGSICPKA GVIFITKKGH KICTDPKLPW VQKHIKNLDA KRNQPSEGAK ALGPKFVIQK LRGNSTKV

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL24/Eotaxin-2/MPIF-2 as determined by LAL method.

Predicted Molecular Mass

10.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Eotaxin 2 Protein is fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration of 50-250 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat CCL24/Eotaxin-2/MPIF-2 Protein

SDS-PAGE: Recombinant Rat CCL24/Eotaxin-2/MPIF-2 Protein [NBP2-35295]

SDS-PAGE: Recombinant Rat CCL24/Eotaxin-2/MPIF-2 Protein [NBP2-35295]

SDS-Page: Rat CCL24/Eotaxin-2/MPIF-2 Protein [NBP2-35295]

Formulation, Preparation and Storage

NBP2-35295
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL24/Eotaxin-2/MPIF-2

CCL24, also named MPIF-2, Eotaxin-2 and Ck beta 6, is a novel CC chemokine recently identified. It is a secreted protein, encoded by CCL24 gene, and produced by activated monocytes and T lymphocytes. CCL24 signals through the CCR3 receptor and has functions of chemotactic activity for resting T-lymphocytes and eosinophils, but none for monocytes and activated lymphocytes. The plasma levels of CCL24 and the aspirin-exacerbated respiratory disease (such as asthma) morbidity rate have positive correlation.

Alternate Names

Eotaxin-2, MPIF-2, MPIF2, CCL24, Eotaxin-2/CCL24

Gene Symbol

CCL24

Additional CCL24/Eotaxin-2/MPIF-2 Products

Product Documents for Recombinant Rat CCL24/Eotaxin-2/MPIF-2 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Rat CCL24/Eotaxin-2/MPIF-2 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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