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Recombinant Human CCL28 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34983

Novus Biologicals, part of Bio-Techne
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NBP2-34983-100ug
NBP2-34983-1mg
NBP2-34983-500ug
NBP2-34983-5ug

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 108 amino acids corresponding to CCL28 Source: E. coli

Uniprot ID: Q9NRJ3

Amino Acid Sequence: SEAILPIASS CCTEVSHHIS RRLLERVNMC RIQRADGDCD LAAVILHVKR RRICVSPHNH TVKQWMKVQA AKKNGKGNVC HRKKHHGKRN SNRAHQGKHE TYGHKTPY

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL28 as determined by LAL method.

Predicted Molecular Mass

12.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CCL28 is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 1.0-10.0 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CCL28 Protein

SDS-PAGE: Recombinant Human CCL28 Protein [NBP2-34983]

SDS-PAGE: Recombinant Human CCL28 Protein [NBP2-34983]

SDS-Page: Human CCL28 Protein [NBP2-34983]

Formulation, Preparation and Storage

NBP2-34983
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL28

CCL28 belongs to CC family chemokine, also named mucosae-associated epithelial chemokine (MEC). The CCL28 cDNA is located on Chr.5 in humans, and the mRNA of CCL28 is highly expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. The amino acids sequence of human and mouse CCL28 shares 83 % identity, when the protein is processed to be the mature form. CCL28 is signaling through CCR3 and CCR10, and chemotactic for resting CD4, CD8 T-cells and eosinophils.

Alternate Names

CCL28, MEC, VIC, MEC/CCL28

Gene Symbol

CCL28

UniProt

Additional CCL28 Products

Product Documents for Recombinant Human CCL28 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human CCL28 Protein

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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