Recombinant Mouse CCL28 Protein

Name: Recombinant Mouse CCL28 Protein
Brand: Novus Biologicals
SKU: NBP2-35169

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35169

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

Q9JIL2

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CCL28 Proteins and Enzymes »

Product Specifications

Description

A single, non-glycosylated polypeptide chain containing 111 amino acids corresponding to CCL28 Source: E. coli

Uniprot ID: Q9JIL2

Amino Acid Sequence: SEAILPMASS CCTEVSHHVS GRLLERVSSC SIQRADGDCD LAAVILHVKR RRICISPHNR TLKQWMRASE VKKNGRENVC SGKKQPSRKD RKGHTTRKHR TRGTHRHEAS R

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL28 as determined by LAL method.

Predicted Molecular Mass

12.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CCL28 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using murine lymphocytes is in a concentration range of 1.0-10.0 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse CCL28 Protein

SDS-PAGE: Recombinant Mouse CCL28 Protein [NBP2-35169]

SDS-Page: Mouse CCL28 Protein [NBP2-35169]

Formulation, Preparation and Storage

NBP2-35169

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL28

CCL28 belongs to CC family chemokine, also named mucosae-associated epithelial chemokine (MEC). The CCL28 mRNA is highly expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. The amino acids sequence of human and mouse CCL28 shares 83% identity, when the protein is processed to be the mature form. CCL28 is signaling through CCR3 and CCR10, and chemotactic for resting CD4, CD8 T-cells and eosinophils.

Alternate Names

CCL28, MEC, VIC, MEC/CCL28

Gene Symbol

CCL28

UniProt