Recombinant Rat CCL4/MIP-1 beta Protein

Name: Recombinant Rat CCL4/MIP-1 beta Protein
Brand: Novus Biologicals
SKU: NBP2-35278

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35278

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Product Specifications
Preparation & Storage
Background
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Key Product Details

Source

E. coli

Accession #

P50230

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CCL4/MIP-1 beta Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 69 amino acids corresponding to CCL4/MIP-1 beta Source: E. coli

Uniprot ID: P50230

Amino Acid Sequence: APIGSDPPTS CCFSYTSRKI HRNFVMDYYE TSSLCSQPAV VFLTKKGRQI CADPSEPWVN EYVNDLELN

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL4/MIP-1 beta as determined by LAL method.

Predicted Molecular Mass

7.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CCL4 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-1000 ng/ml.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35278

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4, 3 % trehalose.
Preservative	Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL4/MIP-1 beta

Chemokine (C-C motif) ligand 4 encoded by the CCL4 gene, also known as macrophage inflammatory protein-1beta (MIP-1beta) is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8+ T cells. In addition, it is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Furthermore, recombinant rat CCL4 contains 69 amino acids and it shares 80 % and 86 % a.a. sequence identity with human and murine CCL4. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.

Alternate Names

Exodus-3, MIP-1 beta, MIP1 beta, MIP-1 beta/CCL4

Gene Symbol

CCL4

UniProt