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Recombinant Mouse CCL4/MIP-1 beta Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35080

Novus Biologicals, part of Bio-Techne
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NBP2-35080-100ug
NBP2-35080-10ug
NBP2-35080-1mg
NBP2-35080-500ug

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 69 amino acids corresponding to CCL4/MIP-1 beta Source: E. coli

Uniprot ID: P14097

Amino Acid Sequence: APMGSDPPTS CCFSYTSRQL HRSFVMDYYE TSSLCSKPAV VFLTKRGRQI CANPSEPWVT EYMSDLELN

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL4/MIP-1 beta as determined by LAL method.

Predicted Molecular Mass

7.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Macrophage Inflammatory Protein 1 beta Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 20-100 ng/ml.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35080
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL4/MIP-1 beta

Chemokine (C-C motif) ligand 4, also known as Macrophage inflammatory protein-1beta (MIP-1beta) is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8+ T cells. In addition, it is a monokine with inflammatory and chemokinetic properties. Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Furthermore, recombinant murine CCL4 contains 69 amino acids and it shares 77 % and 86 % a.a. sequence identity with human and rat CCL4. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.

Alternate Names

Exodus-3, MIP-1 beta, MIP1 beta

Gene Symbol

CCL4

UniProt

Additional CCL4/MIP-1 beta Products

Product Documents for Recombinant Mouse CCL4/MIP-1 beta Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Mouse CCL4/MIP-1 beta Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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