Recombinant Human CCL5/RANTES Protein

Name: Recombinant Human CCL5/RANTES Protein
Brand: Novus Biologicals
SKU: NBP2-35235

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35235

Submit a review

Product Datasheet / COA

Catalog #

Availability

Size / Price

Qty

100% Guarantee

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P13501

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CCL5/RANTES Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 68 amino acids corresponding to CCL5/RANTES Source: E. coli

Uniprot ID: P13501

Amino Acid Sequence: SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL5/RANTES as determined by LAL method.

Predicted Molecular Mass

7.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

RANTES Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 1.0-10 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CCL5/RANTES Protein

SDS-PAGE: Recombinant Human CCL5/RANTES Protein [NBP2-35235]

SDS-Page: Human CCL5/RANTES Protein [NBP2-35235]

Formulation, Preparation and Storage

NBP2-35235

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20mM PB, pH 7.4, 100 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL5/RANTES

Chemokine (C-C motif) ligand 5 (CCL5) also known as RANTES is classified as a chemotactic cytokine or chemokine. It is encoded by the CCL5 gene in humans. CCL5 is chemotactic for T cells, eosinophils, and basophils, and plays an active role in recruiting leukocytes into inflammatory sites which has been reported to be produced by renal tubular epithelium, synovial fibroblasts and selected tumor cells. It also induces the proliferation and activation of certain natural-killer cells to form CHAK cells. Recombinant human CCL5 contains 68 amino acids and it shares 84 % a.a. sequence identity with murine and rat CCL5, respectively.

Alternate Names

RANTES, SISd, CCL5, RANTES/CCL5

Gene Symbol

CCL5

UniProt