Recombinant Mouse CCL7/MCP-3/MARC Protein

Name: Recombinant Mouse CCL7/MCP-3/MARC Protein
Brand: Novus Biologicals
SKU: NBP2-35161

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35161

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Product Specifications
Scientific Data
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Key Product Details

Source

E. coli

Accession #

Q03366

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CCL7/MCP-3/MARC Proteins and Enzymes »

Product Specifications

Description

A single, non-glycosylated polypeptide chain containing 74 amino acids corresponding to CCL7/MCP-3/MARC Source: E. coli

Uniprot ID: Q03366

Amino Acid Sequence: QPDGPNASTC CYVKKQKIPK RNLKSYRRIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTP TPKP

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL7/MCP-3/MARC as determined by LAL method.

Predicted Molecular Mass

8.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

MCP3 Protein is fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 100-300 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse CCL7/MCP-3/MARC Protein

SDS-PAGE: Recombinant Mouse CCL7/MCP-3/MARC Protein [NBP2-35161]

SDS-Page: Mouse CCL7/MCP-3/MARC Protein [NBP2-35161]

Formulation, Preparation and Storage

NBP2-35161

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 2x PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL7/MCP-3/MARC

Murine CCL7, also known as MARC, is belonging to the CC chemokine family. It is encoded by the gene CCL7 and was isolated from a mouse mast cell line after Fc epsilon RI triggering by IgE plus antigen. Sequence comparisons suggest that MARC may be the mouse homologue of the human MCP-3 gene. Similar to the human system, MARC/FIC is postulated the murine MCP-3. The MCP-3 protein family signals through CCR2 expect MCP-1 and possess cross-reacts across species. CCL7/MCP3 has chemotactic fuction for monocytes and eosinophils, but not for neutrophils.

Alternate Names

MARC, MCP-3, CCL7, MCP-3/CCL7

Gene Symbol

CCL7

UniProt