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Recombinant Canine CCL8/MCP-2 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61351

Novus Biologicals, part of Bio-Techne
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NBP2-61351-100ug
NBP2-61351-1mg
NBP2-61351-500ug
NBP2-61351-5ug

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 76 amino acids corresponding to CCL8/MCP-2 Source: E. coli

Uniprot ID: Q68AY9

Amino Acid Sequence: QPDSVSIPIT CCFSMVKRKI PMQKLESYMR ITNSQCPQEA VIFKTKASRE ICADPKQKWV QDYMNHLDQK SQAQKP

Purity

>98%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of CCL8/MCP-2 as determined by LAL method.

Predicted Molecular Mass

8.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/ml.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-61351
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH7.4.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL8/MCP-2

MCP2 and MCP3 are two recently identified monocyte chemotactic proteins produced by human MG63 osteosarcoma cells. Both MCP2 and MCP3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP1. MCP3 also shares 58% amino acid identity with MCP2. Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Alternate Names

MCP-2, MCP2

Gene Symbol

CCL8

Additional CCL8/MCP-2 Products

Product Documents for Recombinant Canine CCL8/MCP-2 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Canine CCL8/MCP-2 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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