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Recombinant Mouse CCL9/10/MIP-1 gamma Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35086

Novus Biologicals, part of Bio-Techne
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NBP2-35086-100ug
NBP2-35086-1mg
NBP2-35086-500ug
NBP2-35086-5ug

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 101 amino acids corresponding to CCL9/10/MIP-1 gamma Source: E. coli

Uniprot ID: P51670

Amino Acid Sequence: QITHATETKE VQSSLKAQQG LEIEMFHMGF QDSSDCCLSY NSRIQCSRFI GYFPTSGGCT RPGIIFISKR GFQVCANPSD RRVQRCIERL EQNSQPRTYK Q

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL9/10/MIP-1 gamma as determined by LAL method.

Predicted Molecular Mass

11.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Macrophage Marker Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 0.1-10 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse CCL9/10/MIP-1 gamma Protein

SDS-PAGE: Recombinant Mouse CCL9/10/MIP-1 gamma Protein [NBP2-35086]

SDS-PAGE: Recombinant Mouse CCL9/10/MIP-1 gamma Protein [NBP2-35086]

SDS-Page: Mouse CCL9/10/MIP-1 gamma Protein [NBP2-35086]

Formulation, Preparation and Storage

NBP2-35086
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL9/10/MIP-1 gamma

Murine MIP-1 gamma (CCL9/10) is belonging to the CC chemokine family. It is encoded by the gene CCL9. MRP-2 (CCL9/10) was isolated from a murine macrophage cell line, RAW 264.7. MRP-2 shows a 51 % sequence identity at the protein level to MRP-1 (C10) and 46% identity to MIP-1-alpha (CCL3). The functional receptor for CCL9/10 has been identified as CCR1. In contrast, other CCR1 ligands, CCL3/MIP-1alpha and CCL5/RANTES are considered to be primary CCR1 ligands in inflammatory responses. CCL9 can activate osteoclasts suggesting an important role for it in bone resorption. Murine MIP-1 gamma (CCL9/10) is 75% amino acid identical to rat CCL9/10.

Alternate Names

CCF18, MIP-1 gamma, MIP1 gamma

Gene Symbol

CCL9/10

UniProt

Additional CCL9/10/MIP-1 gamma Products

Product Documents for Recombinant Mouse CCL9/10/MIP-1 gamma Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Mouse CCL9/10/MIP-1 gamma Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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