Recombinant Human CD40 Ligand/TNFSF5 Protein

Name: Recombinant Human CD40 Ligand/TNFSF5 Protein
Brand: Novus Biologicals
SKU: NBP2-35244

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35244

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

P29965

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CD40 Ligand/TNFSF5 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 149 amino acids corresponding to CD40 Ligand/TNFSF5 Source: E. coli

Uniprot ID: P29965

Amino Acid Sequence: MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CD40 Ligand/TNFSF5 as determined by LAL method.

Predicted Molecular Mass

16.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 5-10 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CD40 Ligand/TNFSF5 Protein

SDS-PAGE: Recombinant Human CD40 Ligand/TNFSF5 Protein [NBP2-35244]

SDS-Page: Recombinant Human CD40 Ligand/TNFSF5 Protein [NBP2-35244]

Formulation, Preparation and Storage

NBP2-35244

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.0.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-0.3mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CD40 Ligand/TNFSF5

CD40 ligand is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF family and can be cleaved into two chains: the CD40 ligand membrane form and CD40 ligand soluble form. It is primarily expressed on activated CD4+ T lymphocytes, and also found in other types of cells, like NK cells, mast cells, basophils and eosinophils. In total CD40 ligand has three binding partners: CD40, alpha 5 beta 1 integrin and alpha IIb beta3. It mediates a range of activities on B cells including induction of activation-associated surface antigen, entry into cell cycle, isotype switching and Ig secretion and memory generation. CD40- CD40L interaction also plays important roles in monocyte activation and dendritic cell maturation.

Alternate Names

CD154, CD40L, CD40LG, gp39, TNFSF5, TRAP, CD40 Ligand/TNFSF5

Gene Symbol

CD40LG

UniProt