Recombinant Rat CDNF Protein

Name: Recombinant Rat CDNF Protein
Brand: Novus Biologicals
SKU: NBP2-35246

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35246

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CDNF Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 163 amino acids corresponding to CDNF Source: E. coli

Uniprot ID: P0C5I0

Amino Acid Sequence: QGLEAGVRSR ADCEVCKEFL NRFYNSLLTR GIDFSVDTIE EELISFCADT KGKENRLCYY LGATKDSATK ILGEVTRPMS VHMPTVKICE KLKKMDSQIC ELKYEKKLDL ESVDLWKMRV AELKQILHSW GEECRACAEK HDYVNLIKEL APKYVETRPQ TEL

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of CDNF as determined by LAL method.

Predicted Molecular Mass

18.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CDNF protein is fully biologically active when compared to standard. It is able to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons when immobilized at 5 - 25 ug/mL on a nitrocellulose-coated microplate

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat CDNF Protein

SDS-PAGE: Recombinant Rat CDNF Protein [NBP2-35246]

SDS-Page: Rat CDNF Protein [NBP2-35246]

Formulation, Preparation and Storage

NBP2-35246

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CDNF

Cerebral dopamine neurotrophic factor (CDNF), also known as ARMET-like protein 1, is a protein encoded by the CDNF gene and it is widely expressed in neuronal and non-neuronal tissues. The cerebral dopamine neurotrophic factor (CDNF) also is a novel neurotrophic factor with strong trophic activity on dopaminergic neurons comparable to that of glial cell line-derived neurotrophic factor (GDNF). By research, CDNF prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons and it might be beneficial for the treatment of parkinson's disease. Recombinant rat CDNF contains 163 amino acid residues and it shares 83 % and 87 % a.a. sequence identity with human and murine CDNF.

Long Name

Conserved Dopamine Neurotrophic Factor

Alternate Names

ARMETL1

Gene Symbol

CDNF

Additional CDNF Products

Product Documents for Recombinant Rat CDNF Protein

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COA

Product Specific Notices for Recombinant Rat CDNF Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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