Recombinant Human CHMP4A His Protein

Name: Recombinant Human CHMP4A His Protein
Brand: Novus Biologicals
SKU: NBP1-78875

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78875

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Technical Data
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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all CHMP4A Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-265 of Human CHMP4A

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSRRRPEDGL GKAGPCVMRH HPPRSKAEVW RTLRGGGGRG ELAMSGLGRL FGKGKKEKGP TPEEAIQKLK ETEKILIKKQ EFLEQKIQQE LQTAKKYGTK NKRAALQALR RKKRFEQQLA QTDGTLSTLE FQREAIENAT TNAEVLRTME LAAQSMKKAY QDMDIDKVDE LMTDITEQQE VAQQISDAIS RPMGFGDDVD EDELLEELEE LEQEELAQEL LNVGDKEEEP SVKLPSVPST HLPAGPAPKV DEDEEALKQL AEWVS

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

31.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CHMP4A His Protein

SDS-PAGE: Recombinant Human CHMP4A His Protein [NBP1-78875]

SDS-Page: Recombinant Human CHMP4A His Protein [NBP1-78875] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-78875

Formulation	20 mM Tris-HCl buffer (pH 8.0), 2 mM DTT, 50% glycerol, 200 mM NaCl, 0.1 mM PMSF, 1 mM EDTA
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CHMP4A

CHMP4A (Chromatin-modifying protein 4a) belongs to the SNF7 family and functions as chromatin-modifying proteins. It probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. Also during HIV-1 infection, the virus uses the ESCRT-III complex to mediate budding and exocytosis of viral proteins via the association of CHMP4 and a protein recruited by HIV-1 p6, which is present in viral Gag assembly and budding. CHMP4A expressed at higher level in heart, kidney, liver and skeletal muscle. Recombinant human CHMP4A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

C14orf123, charged multivesicular body protein 4a, CHMP4, CHMP4a, CHMP4B, chromatin modifying protein 4A, Chromatin-modifying protein 4a, chromosome 14 open reading frame 123, FLJ61658, hSnf-1, HSPC134, hVps32-1, MGC142093, MGC142095, SHAX2, SNF7, SNF7 homolog associated with Alix-2, Snf7 homologue associated with Alix 2, SNF7-1, Vacuolar protein sorting-associated protein 32-1, Vps32-1, VPS32A

Gene Symbol

CHMP4A

Additional CHMP4A Products

Product Documents for Recombinant Human CHMP4A His Protein

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COA

Product Specific Notices for Recombinant Human CHMP4A His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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